Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil
نویسندگان
چکیده
Crop straw contains huge amounts of exploitable energy, and efficient biomass degradation measures have attracted worldwide attention. Mining strains with high yields cellulose-degrading enzymes is great significance for developing clean energy industrial production related enzymes. In this study, we reported a high-quality genome sequence Bacillus velezensis SSF6 strain using high-throughput sequencing technology (Illumina PE150 PacBio) assessed its lignocellulose potential. The results demonstrated that the B. was 3.89 Mb contained 4,015 genes, which 2,972, 3,831 158 genes were annotated in COGs (Clusters Orthologous Groups), KEGG (Kyoto Encyclopedia Genes Genomes) CAZyme (Carbohydrate-Active enZymes) databases, respectively, large number to carbohydrate metabolism. Furthermore, has cellulose capacity, filter paper assay (FPA) an exoglucanase activity 64.48 ± 0.28 78.59 0.42 U/mL, respectively. Comparative genomic analysis depicted richer hydrolase gene. conclusion, ability revealed by determination cellulase activity, laid foundation further bioconversion.
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2023
ISSN: ['1664-302X']
DOI: https://doi.org/10.3389/fmicb.2023.1167293